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KMID : 0857020060210010302
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Kosin Medical Journal
2006 Volume.21 No. 1 p.302 ~ p.309
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Effects of recombinant erythropoietin and interaction with brain-derived neurotrophic factor in primary cultured cortical neuron in chronic hypoxia.
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Kim Hyun
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Abstract
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Chronic hypoxia has been associated with change in neurovascular behavior, mediated, in part, by erythropoietin (EPO) and brain-derived neurotrophic factor (BDNF). EPO, a hematopoietic growth factor, and BDNF could act as a neurotrophic factor. In the present study, I investigated the characteristics of BDNF and EPO expressions by primary cultured cortical neuron in vivo and in vitro and tested the hypothesis that EPO and BDNF serve protective functions in chronic hypoxia. In addition to expressing EPO, neurons increased their expression of BDNF, markedly with treatment of recombinant EPO under hypoxia. Also exogenous EPO increased phosphorylation of trkB, receptor of BDNF. Finally, exogenous EPO decreased apoptosis of cultured neurons as evaluated by expression of PARP via its own function and induction of BDNF expression. These data support and interaction with EPO and BDNF in maintenance of primary cultured cortical neuron in chronic hypoxia.
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KEYWORD
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erythropoietin (EPO), brain-derived neurotrophic factor (BDNF), trkB, chronic hypoxia
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